Molecular Histology and Fluorescence Imaging Services
State-of-the-art histological analysis of tissues and fluorescent imaging are important aspects of the research for 老虎机攻略 investigators. Using histological techniques, it is possible to monitor tissue remodeling and cellular infiltration as a result of disease or toxicant exposure. The inclusion of fluorescent imaging in research allows examination of the subcellular distribution of molecules and potential interactions between molecules of interest. Consultation on the design, implementation and interpretation of imaging and histological data is a key component of this shared resource facility.
Imaging:
The MHFI Core’s primary light microscope is an upright Nikon E-800, with Brightfield, DIC and fluorescence capabilities. Digital images are generated with an Olympus DP71 CCD camera. The cellSens software includes Extended Focus Image (EFI) to create a manual z stack as a single image and Multiple Image Array (MIA) for image stitching.
Objectives include:
- 4X/0.13 NA Plan Fluor
- 10X/0.30 NA Plan Fluor
- 20X/0.50 NA Plan Fluor
- 40X/0.75 NA Plan Fluor
- 60X/1.40 NA oil Plan-Apochromat
- 100X/1.40 NA oil Plan-Apochromat
Fluorescence Filter sets: DAPI, FITC, Texas Red, and FITC/TR
The MHFI Olympus FV1000 laser scanning confocal microscopy system is capable of obtaining multicolor, 3D images of living cells in a controlled environment. It can also perform spectral unmixing to allow resolution of fluorescence overlap and Total Internal Reflection Fluorescence (TIRF) microscopy. Laser lines available include 405, 458, 488, 515, 559 and 635nm.
Confocal laser scanning microscopy permits high resolution optical sectioning without compromising specimen integrity. Some advantages of using confocal microscopy include:
- Elimination of out of focus planes, yielding sharper images than conventional fluorescence microscopy.
- Analysis of thick specimens.
- Computer controlled microscopy, permitting fine focus control and digitization of images.
- Collection of data sets for subsequent three dimensional reconstruction.
- Ability to collect two images simultaneously from specimens labeled with multiple fluorochromes.
Olympus:
Support:
Assistance in experimental design, data analysis and developing new methods is available by appointment with the Staff Scientist.
Rate Charges
| PROCEDURE | PRICE UNIT |
|---|---|
| HISTOLOGY | |
| Processing and embedding paraffin blocks*(Min $25/run, Max charge $40/run) | $4/ block independent use $6/ block operator rate |
| Depar/ Dehydration Series- manual | $5/run independent use $10/run operator rate |
| Paraffin sections | |
| Unstained sections | $4/slide operator rate |
| Hematoxylin & Eosin stain* | $25/ run independent use |
| $8/slide operator rate, min $25 | |
| Special staining techniques | $10/slide operator supplies reagents |
| Microtome use* | $10/hr independent use $35/ hr operator rate |
| Use of cryostat* | $10/hr independent use $35/hr operator rate |
| Frozen sections | $10/hr slide operator rate |
| Immunohistochemistry (plus cost of primary antibody) | $35/hour |
| IMAGING | |
| Confocal Imaging* | $15/hr independent use $50/hr operator rate |
| Fluorescence/ Transmission Imaging | $10/hr independent use $35/hr operator rate |