Macromolecular Crystallography [ISB 016A]
- Rigaku MicroMax 007HF X-ray generator with VariMax HighFlux optic. Pilates Dectris 200K detector and AFC Kappa 4-circle goniometer
- Oxford liquid nitrogen crystal cryo-cooling system with auto refill
- Art Robbins Instruments GRYPHON liquid-handling crystallization robot
- Scorpion Matrix maker for custom crystallization grid preparation
- Crystallization kits, including Hampton, Qiagen, JCSG, and Wizard Suites
- JANSi visible/UV fluorescence digital microscope for crystal viewing and documentation
- Olympus microscope with variable objectives for crystal viewing and documentation
- Zeiss Stemi SU8 stereomicroscope for crystal harvesting
- 3 low-vibration incubators (4-20°C) for storage of crystallization experiments
- GEForce RTX 2080 4-node GPU workstation for cryoEM and crystallographic analysis
- Linux workstation, installed with crystallographic software suites, is available for in-house and remote diffraction data processing and structure determination
- HKL2000 and CrysAlisPRO software for data processing and scaling
- CCP4, PHENIX, COOT, and PYMOL software packages for crystal structure determination and visualization
- ATSAS software package for small-angle X-ray scattering data analysis
- RELION, CryoSPARC, EMAN2 and IMOD software for Cryo-EM single particle structure determination and tomography
GENERAL LABORATORY EQUIPMENT (ISB 016B and 015)
- Thermo Scientific Barnsted NanoPure water purification system
- Beckman Coulter Optima XE-90 ultracentrifuge with 2 Beckman Coulter Ti45 rotors
- Sorvall RC6+ Floor centrifuge with Fiberlite F10 4x10000 LEX and Fiberlite F21-8x50Y rotors
- Sorvall RT7 bench-top centrifuge
- BenchMark bench-top incubator/shaker
- LabNet PrismR benchtop refrigerated microcentrifuge
- Thermo Scientific Legend Macro 21 PCR cycler
- 3-door 4oC Deli fridge
- Three 4°C laboratory refrigerators (one dedicated to ISB016C)
- -20°C laboratory freezer
- VWR -80°C laboratory freezer
Bacterial Protein Expression [ISB 015B, 016B]
- Two stacked Innova 4430 refrigerated floor shakers for bacterial growth
- Barnstead/Labline MaxQ/400 and benchtop orbital shaker
- Brunswick Bioflo/Celligen 115 fermenter with Eppendorf 7L vessel and Thermoflex 1400 recirculating water bath
- Thermo Fisher Isotemp incubator for bacterial growth
Eukaryotic Cell Protein Expression [ISB 016C]
- SterilGard III Advance Class II Biosafety Cabinet
- Two stackable Innova 4430 temperature-controlled incubator shakers for maintenance and scale-up insect cell growth
- Thermo Forma Direct Heart CO2 Incubator with HEPA filter for batch mammalian cell culture, equipped with orbital shakers
- Thermo Forma MIDI 40 temperature controlled CO2 incubator for mammalian cell passaging
- Nikon Eclipse Ti-SM inverted microscope
- Left Technology Countess II cell counter
- Binder Drying Oven for baking sterilized culture flask
Protein Purification [ISB 016B]
- 2 GE AKTA-Pure fast performance Chromatography System for high-throughput protein purification, with 1 Shimadzu RF-20Axs Prominence fluorescence detector
- Avestin Emulsiflex C5 High Pressure Homogenizer for cell lysis
- Large collection of commercial pre-packed and in-house packed affinity, ion-exchange, hydrophobic interaction and size-exclusion chromatographic columns
Protein characterization [ISB 016B, ISB 207]
- Microcal VP-ITC Calorimeter for determination of binding constants of protein-protein or protein-ligand interactions
- Two BioRad Mini Protean slab gel electrophoresis units with power source
- Thermo Scientific NanoDrop One microspectrophotometer
- Two BioRad Transblot transfer cells for Western blots
- Wyatt TREOS-II multi-angle light scattering detector and Optilab T-rEX Index of Refraction monitor connected in-line to output of Superdex 200 column mounted on an Agilent 1100 series HPLC system
- Biacore X100 surface plasmon resonance detector for protein-ligand binding kinetics and affinity
- Nano-temper Tycho NT.6 thermo-fluorescence detector for determination of protein stability
- Perkin Elmer LS55 luminescence spectrometer